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期刊论文 41

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关键词

CP）；符号间干扰（inter symbol interference， ISI）；载波间干扰（inter carrier interference 1

ICI）；最大似然估计（maximum likelihood estimation 1

MLE） 1

OFDM）；快速傅立叶变换（Fast Fourier transform， FFT）；循环前缀（cyclic prefix 1

RNA干扰（RNAi） 1

SARS-CoV-2干扰 1

Z干扰信道；非正则信号；和速率；帕累托边界；协方差；伪协方差 1

传输性能分析模型 1

佩戴口罩 1

信息流分析；业务流程执行语言；Petri网；干扰性 1

地下无线电通信 1

基于RNA的生物防治 1

多用户干扰 1

大规模多输入多输出；全双工；小蜂窝；无线回程链路；分布式算法 1

大豆臭虫（SSB） 1

导电陶瓷复合材料 1

弱信号接收 1

抗雷电脉冲干扰 1

无人机；泊松点过程；多点协同；干扰统计特性；覆盖性能 1

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The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

《医学前沿（英文）》 2009年 第3卷 第4期   页码 384-389 doi: 10.1007/s11684-009-0070-1

摘要： In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis. The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000. The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. The cell cycle was analyzed by using DNA content analysis by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain. CDK1 gene was successfully silenced by its siRNA, and the CDK1 protein expression level was decreased significantly, especially from 48thh to 60thh after transfection. The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G/M phase. There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60h. More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells. The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G/M phase but did not induce apoptosis.

关键词： cyclin-dependent kinase1     siRNA interference     cell cycle     apoptosis    

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Elastoplastic frictional contact problem study on interference fits of compressor

ZHANG Hongwu, WU Changhua, LIAO Aihua

《能源前沿（英文）》 2008年 第2卷 第3期   页码 313-319 doi: 10.1007/s11708-008-0026-1

摘要： The FE parametric quadratic programming (PQP) method developed from the parametric variational principle (PVP) was used for the analysis of the stress distribution of the 3D elastoplastic frictional contact of an impeller-shaft sleeve-shaft. A locomotive-type turbocharger compressor with 24 blades under combined centrifugal and interference-fit loading was considered in the numerical analysis. The solution of elastoplastic frictional contact problems belongs to unspecified boundary problems where the interaction between two kinds of nonlinearities should occur. To save time in the numerical computation, a multi-substructure technique was adopted in the structural modeling. The effect of fit tolerance, wall thickness of the shaft sleeve, and rotational speed on the contact stress was discussed in detail in the numerical computation. To decrease the difficulty of the assembly process and ensure the safety of the working state, the amount of interference between the shaft sleeve and shaft by press-fitting should be controlled strictly to avoid the rapid increase of contact stress. The numerical results show that the algorithm has high accuracy and good convergence. The study can be referred to in deciding the proper fit tolerance and improving the design and manufacturing technology of compressor impellers.

关键词： computation     thickness     interference-fit     interference     unspecified    

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Laser interference fabrication of large-area functional periodic structure surface

Lei WANG, Zi-Han WANG, Yan-Hao YU, Hong-Bo SUN

《机械工程前沿（英文）》 2018年 第13卷 第4期   页码 493-503 doi: 10.1007/s11465-018-0507-9

摘要：

Functional periodic structures have attracted significant interest due to their natural capabilities in regulating surface energy, surface effective refractive index, and diffraction. Several technologies are used for the fabrication of these functional structures. The laser interference technique in particular has received attention because of its simplicity, low cost, and high-efficiency fabrication of large-area, micro/nanometer-scale, and periodically patterned structures in air conditions. Here, we reviewed the work on laser interference fabrication of large-area functional periodic structures for antireflection, self-cleaning, and superhydrophobicity based on our past and current research. For the common cases, four-beam interference and multi-exposure of two-beam interference were emphasized for their setup, structure diversity, and various applications for antireflection, self-cleaning, and superhydrophobicity. The relations between multi-beam interference and multi-exposure of two-beam interference were compared theoretically and experimentally. Nanostructures as a template for growing nanocrystals were also shown to present future possible applications in surface chemical control. Perspectives on future directions and applications for laser interference were presented.

关键词： laser interference     four-beam interference     multi-exposure of two-beam interference     additive fabrication    

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Influence of Survivin-targeted siRNA on the biological features of colorectal carcinoma cells

XIONG Ying, GUO Wen, LI Ting, LI Ke

《医学前沿（英文）》 2007年 第1卷 第3期   页码 304-307 doi: 10.1007/s11684-007-0058-7

摘要： The transient transfection of survivin-targeted siRNA to Lovo cells and its influence on the biological features were studied. Two pairs of 19 base pairs (bp) siRNA-specific targeted survivin gene were designed and synthesized by transcription (Survivin-1, Survivin-2). After transient transfection of the two survivin-targeted siRNAs to Lovo cells by Lipofectamine™ 2000, the expression of survivin mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Apoptosis was detected by flow cytometry and cell proliferation was evaluated by MTT assay. We found that the expression levels of survivin mRNA of the two RNAi groups (Survivin-1 group and Survivin-2 group) respectively decreased by 70% and 39.1% compared with the control Lovo’s. Seventy-two hours after transfection, apoptosis rates of the two RNAi groups were 21.51% and 26.28%, both of which were higher than control Lovo’s (9.03%). The results at 72 h after transfection were that the optical density (OD) at 490 nm of the two RNAi groups was 0.581±0.070 and 0.681±0.104, both of which were much lower than the control Lovo’s (2.060±0.272). Based on the results, we can draw a conclusion that the two survivin-targeted siRNAs successfully suppressed the expression of survivin mRNA, inhibited cell growth and induce cell apoptosis. It provides a powerful evidence for colorectal carcinoma gene therapy.

关键词： control     therapy     influence     Survivin-1     colorectal carcinoma    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

《化学科学与工程前沿（英文）》 2017年 第11卷 第4期   页码 663-675 doi: 10.1007/s11705-017-1623-5

摘要： Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

关键词： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

《医学前沿（英文）》 2009年 第3卷 第1期   页码 57-60 doi: 10.1007/s11684-009-0003-z

摘要： Leptin resistance is a main mechanism of acquired childhood obesity, and the suppression of long form of leptin receptor (OBRb) gene expression in diet-induced obese rats indicates that the down-regulation of OBRb gene expression plays a pivotal role in the mechanism of leptin resistance. The aim of the present study was to construct the lentiviral RNA interference (RNAi) vector of rat OBRb gene and evaluate the effects of siRNA on silencing OBRb gene expression. The target sequence of siRNA-OBRb was designed, and the complementary DNA containing both sense and antisense oligonucleotides was synthesized. After phosphorylation and annealing, these double-stranded DNA was cloned to pRNA-lentivector-VGFP to construct pRNA-Lenti-OBRb-VGFP recombinants with U6-containing promoter, target sequence and Poly III terminator. Then, the products were confirmed by electrophoresis and sequencing analysis, and the effects of RNAi on reducing gene expression were further confirmed by real-time polymerase chain reaction in transfected rat glioma cells expressing OBRb. The target sequence of siRNA-OBRb was successfully cloned to pRNA-lentivector-VGFP, and the RNAi protocol specifically reduced the expression of OBRb mRNA by approximately 80% compared with controls in transfected rat glioma cells. The successful construction of rat lentivirus vectors expressing OBRb-specific shRNA may be useful for further investigation .

关键词： receptors     leptin     RNA interference     lentivirus vector    

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with multi-walled carbon nanotubes: preparation, characterization, and application for electromagnetic interference

Jian LI,Caichao WAN

《农业科学与工程前沿（英文）》 2015年 第2卷 第4期   页码 341-346 doi: 10.15302/J-FASE-2015082

摘要： Electromagnetic wave pollution has attracted extensive attention because of its ability to affect the operation of electronic machinery and endanger human health. In this work, the environmentally-friendly hybrid aerogels consisting of cellulose and multi-walled carbon nanotubes (MWCNTs) were fabricated. The aerogels have a low bulk density of 58.17 mg·cm . The incorporation of MWCNTs leads to an improvement in the thermal stability. In addition, the aerogels show a high electromagnetic interference (EMI) value of 19.4 dB. Meanwhile, the absorption-dominant shielding mechanism helps a lot to reduce secondary radiation, which is beneficial to develop novel eco-friendly EMI shielding materials.

关键词： cellulose aerogels     carbon nanotubes     electromagnetic interference shielding     composites    

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Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

《机械工程前沿（英文）》 2018年 第13卷 第3期   页码 329-353 doi: 10.1007/s11465-018-0466-1

摘要：

Electromagnetic interference (EMI) causes electromechanical damage to the motors and degrades the reliability of variable-frequency drive (VFD) systems. Unlike fundamental frequency components in motor drive systems, high-frequency EMI noise, coupled with the parasitic parameters of the trough system, are difficult to analyze and reduce. In this article, EMI modeling techniques for different function units in a VFD system, including induction motors, motor bearings, and rectifier-inverters, are reviewed and evaluated in terms of applied frequency range, model parameterization, and model accuracy. The EMI models for the motors are categorized based on modeling techniques and model topologies. Motor bearing and shaft models are also reviewed, and techniques that are used to eliminate bearing current are evaluated. Modeling techniques for conventional rectifier-inverter systems are also summarized. EMI noise suppression techniques, including passive filter, Wheatstone bridge balance, active filter, and optimized modulation, are reviewed and compared based on the VFD system models.

关键词： variable-frequency drive (VFD)     electromagnetic interference (EMI)     motor drive modeling     EMI noise suppression    

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Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 303-308 doi: 10.1007/s11684-009-0060-3

摘要： The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H (non-transfected Hela cells), H (pGenesil-1 empty plasmid-transfected Hela cells), and H (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O) and normoxia for 48h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch test and the invasion assay were applied to examine the invasion in each group. The expression of HIF-1α, E-cadherin, and β-catenin in tumor grafts was assayed immunohistochemically. Western blot results revealed that the bands of HIF-1α, E-cadherin, β-catenin, and actin proteins were detected in the H and H groups under hypoxia for 48h. The expression of E-cadherin, β-catenin, and actin was detected in the H group under hypoxia for 48h, and normoxia. In the H, H, and H groups under normoxia, and the H group under hypoxia for 48h, no expression of HIF-1α was detectable. The scratch test showed that the invasive ability in the H group was significantly alleviated. Immunohistochemically, it was found that there was a significant difference in the expression of HIF-1α, E-cadherin, and β-catenin between the H and H groups (<0.05), but the difference was not significant between the H and H groups. It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins (E-cadherin, β-catenin, and actin) depend on HIF-1α.

关键词： hypoxia     actin     β     -catenin     E-cadherin     invasion    

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Experimental study of aerodynamic interference effects on aerostatic coefficients of twin deck bridges

Zhiwen LIU , Zhengqing CHEN , Gao LIU , Xinpeng SHAO ,

《结构与土木工程前沿（英文）》 2009年 第3卷 第3期   页码 292-298 doi: 10.1007/s11709-009-0048-8

摘要： The aerodynamic interference effects on aerostatic coefficients of twin deck bridges with large span were investigated in detail by means of wind tunnel test. The distances between the twin decks and wind attack angles were changed during the wind tunnel test to study the effects on aerodynamic interferences of aerostatic coefficients of twin decks. The research results have shown that the drag coefficients of the leeward deck are much smaller than that of a single leeward deck. The drag coefficients of a windward deck decrease slightly compared with that of a single deck. The lift and torque coefficients of windward and leeward decks are also affected slightly by the aerodynamic interference of twin decks. And the aerodynamic interference effects on lift and torque coefficients of twin decks can be neglected.

关键词： twin decks     aerodynamic interference effects     aerostatic coefficients     wind tunnel test    

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Calculation method of radial stress and deformation on conic threaded connections with interference fit

Shoujun CHEN, Qiang LI, Qi AN,

《机械工程前沿（英文）》 2010年 第5卷 第3期   页码 302-307 doi: 10.1007/s11465-010-0018-9

摘要： This paper presents a calculation method for radial stress distribution and deformation on conic threaded connections with interference fit. Based on elastic mechanics, a new calculation model is established using the thick-walled-cylindrical theory. A sample calculation for API 88.9 mm conic threaded connection indicates that the method proposed in this paper is reasonable, and the finite element analysis (FEA) method is used to validate the proposed method. The results obtained by the proposed method and FEA method are identical. The model offers a new way of calculating the radial stress and deformation on conic thread connections with interference fit.

关键词： conic threaded connections     interference fit     radial stress     deformation     finite element analysis (FEA)    

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Design of packing cup interference fit value of hypercompressors for low density polyethylene production

Da LEI,Xuehong LI,Yun LI,Xiwen REN

《能源前沿（英文）》 2019年 第13卷 第1期   页码 107-113 doi: 10.1007/s11708-017-0450-1

摘要： The hypercompressor is one of the core facilities in low density polyethylene production, with a discharge pressure of approximately 300 MPa. A packing cup is the basic unit of cylinder packing, assembled by the interference fit between an inner cup and an outer cup. Because the shrink-fitting prestresses the packing cup, serious design is needed to gain a favorable stress state, for example, a tri-axial compressive stress state. The traditional method of designing the interference fit value for packing cups depends on the shrink-fit theory for thick-walled cylinder subject to internal and external pressure. According to the traditional method, critical points are at the inner radii of the inner and external cup. In this study, the finite element method (FEM) has been implemented to determine a more accurate stress level of packing cups. Different critical points have been found at the edge of lapped sealing surfaces between two adjacent packing cups. The maximum Von Mises equivalent stress in a packing cup increases after a decline with the rise of the interference fit value. The maximum equivalent stress initially occurs at the bore of the inner cup, then at the edge of lapped mating surfaces, and finally at the bore of the outer cup, as the interference radius increases. The traditional method neglects the influence of axial preloading on the interference mating pressure. As a result, it predicts a lower equivalent stress at the bore of the external cup. A higher interference fit value accepted by the traditional method may not be feasible as it might already make packing cups yield at the edge of mating surfaces or the bore of the external cup. Along with fatigue analysis, the feasible range of interference fit value has been modified by utilizing FEM. The modified range tends to be narrower and safer than the one derived from the traditional method, after getting rid of shrink-fit values that could result in yielding in a real packing cup.

关键词： interference fit value     packing cup     hypercompressor     finite element method (FEM)    

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Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

《医学前沿（英文）》 2008年 第2卷 第3期   页码 235-238 doi: 10.1007/s11684-008-0044-8

摘要： The aim of this study is to investigate the effect of tyrosine kinase Src on Tyrosine 307(Y307) phosphorylation, protein phosphatase 2A (PP2A) activity, and on tau phosphorylation. Specific Src siRNA was transfected into cultured mouse neuroblastoma N2a cells to inhibit the expression of Src protein, and the phosphorylation levels of PP2A Y307 and tau at different sites, as well as PP2A activity were detected at different time points after siRNA transfection. Twelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreased PP2A Y307 phosphorylation. However, the total PP2A protein level was also decreased, together with a decreased PP2A activity. Tau was hyperphosphorylated at the Ser198/199/202 sites. Multiple factors may be involved in the cellular regulation of PP2A activity. Inhibiting Src expression could induce inactivation of PP2A and tau hyperphosphorylation.

关键词： hyperphosphorylation     PP2A activity     cellular regulation     siRNA     siRNA transfection    

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Damage propagation and strength prediction of a single-lap interference-fit laminate structure

Peng ZOU, Xiangming CHEN, Hao CHEN, Guanhua XU

《机械工程前沿（英文）》 2020年 第15卷 第4期   页码 558-570 doi: 10.1007/s11465-020-0591-5

摘要： Experimental and finite element research was conducted on the bolted interference fit of a single-lap laminated structure to reveal the damage propagation mechanism and strength change law. A typical single-lap statically loading experiment was performed, and a finite element damage prediction model was built based on intralaminar progress damage theory. The model was programmed with a user subroutine and an interlaminar cohesive zone method. The deformation and damage propagation of the specimen were analyzed, and the failure mechanism of intralaminar and interlaminar damage during loading was discussed. The effect of secondary bending moment on load translation and damage distribution was revealed. The experimental and simulated load–displacement curves were compared to validate the developed model’s reliability, and the ultimate bearing strengths under different fit percentages were predicted. An optimal percentage was also recommended.

关键词： single-lap     interference fit     secondary bending moment     damage mechanism     bearing strength    

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Impact of siRNA targeting pirh2 on proliferation and cell cycle control of the lung adenocarcinoma cell

SU Yuan, JIN Yang, ZHANG Xiaoju, ZHOU Qiong, BAI Ming, ZHU Liping

《医学前沿（英文）》 2007年 第1卷 第4期   页码 359-363 doi: 10.1007/s11684-007-0069-4

摘要： The aim of this study was to investigate the role of pirh2 (p53-induced RING-H2) protein in the proliferation, apoptosis and cell cycle control of the lung cancer cell line A549. Pirh2 expression was detected by immunofluorescence, Western blot analysis and real-time polymerase chain reaction (PCR). Cell proliferation was assessed by cell counting kit-8 (CCK-8). Cell cycle control and apoptosis were analyzed by flow cytometry. The results showed that pirh2 was expressed in the cytoplasm of A549 cells. The inhibition of pirh2 expression by siRNA (psiRNA-pirh2) resulted in reduced cell proliferation and increased apoptosis. In addition, the number of G/G phase cells was increased but G/M cells were not affected significantly. Taken together, the inhibition of pirh2 expression in the lung adenocarcinoma cell line A549 resulted in reduced tumor cell growth via the inhibition of cell proliferation, the activation of apoptosis and the interruption of cell cycle transition.

关键词： control     interruption     cytoplasm     number     growth    

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标题 作者 时间 类型 操作

The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

期刊论文

Elastoplastic frictional contact problem study on interference fits of compressor

ZHANG Hongwu, WU Changhua, LIAO Aihua

期刊论文

Laser interference fabrication of large-area functional periodic structure surface

Lei WANG, Zi-Han WANG, Yan-Hao YU, Hong-Bo SUN

期刊论文

Influence of Survivin-targeted siRNA on the biological features of colorectal carcinoma cells

XIONG Ying, GUO Wen, LI Ting, LI Ke

期刊论文

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

期刊论文

Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

期刊论文

with multi-walled carbon nanotubes: preparation, characterization, and application for electromagnetic interference

Jian LI,Caichao WAN

期刊论文

Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

期刊论文

Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

期刊论文

Experimental study of aerodynamic interference effects on aerostatic coefficients of twin deck bridges

Zhiwen LIU , Zhengqing CHEN , Gao LIU , Xinpeng SHAO ,

期刊论文

Calculation method of radial stress and deformation on conic threaded connections with interference fit

Shoujun CHEN, Qiang LI, Qi AN,

期刊论文

Design of packing cup interference fit value of hypercompressors for low density polyethylene production

Da LEI,Xuehong LI,Yun LI,Xiwen REN

期刊论文

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

期刊论文

Damage propagation and strength prediction of a single-lap interference-fit laminate structure

Peng ZOU, Xiangming CHEN, Hao CHEN, Guanhua XU

期刊论文

Impact of siRNA targeting pirh2 on proliferation and cell cycle control of the lung adenocarcinoma cell

SU Yuan, JIN Yang, ZHANG Xiaoju, ZHOU Qiong, BAI Ming, ZHU Liping

期刊论文